Fluorescence Microscopy (1210)
This three day course is designed to teach the fundamentals of fluorescence microscopy and to provide an overview of specialized methods and applications.
There is no prerequisite for this course.
COURSE OUTLINE & SYLLABUS
Students will acquire the basic knowledge and practical skills to apply fluorescence methods in their areas of interest. The majority of the course will be hands-on demonstration and training in the practice of sample preparation for epifluorescence microscopy.
There is no prerequisite for this course.
• Introduction to the theory and practice of epifluorescence microscopy
• Introductory fluorescence investigations
• Microscopes for fluorescence
• Filter sets and cleaning
• Pitfalls and shortcomings of fluorescence microscopy
• Intrinsic, general and specific fluorescence probes
• Viability probes
• Sample preparation
• Dye loading
• Problem solving
• Resources, materials and probes
Note: This course runs 8:00 am to 5:00 pm on all three days